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45-and 70-base DNA supramolecular polymerizations on quartz crystal microbalance biosensor ArchiMer
Lazerges, Mathieu; Perrot, Hubert; Rabehagasoa, Niriniony; Antoine, Elisabeth; Compere, Chantal.
Supramolecular polymerizations of 45- and 70- base DNAs on the surface of an in-situ time-resolved 27 MHz quartz crystal microbalance biosensor.
Tipo: Text Palavras-chave: Sensor; Design; Kinetics; Immobilization; Hybridization.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-1710.pdf
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Thiol- and Biotin-Labeled Probes for Oligonucleotide Quartz Crystal Microbalance Biosensors of Microalga Alexandrium Minutum ArchiMer
Lazerges, Mathieu; Perrot, Hubert; Rabehagasoa, Niriniony; Compere, Chantal.
Two quartz crystal microbalance oligonucleotide biosensors of a toxic microalga gene sequence (Alexandrium Minutum) have been designed. Grafting on a gold surface of 20-base thiol- or biotin-labeled probe, and selective hybridization with the complementary 20-base target, have been monitored in situ with a 27 MHz quartz crystal microbalance under controlled hydrodynamic conditions. The frequency of the set up is stable to within a few hertz, corresponding to the nanogram scale, for three hour experiments. DNA recognition by the two biosensors is efficient and selective. Hybridization kinetic curves indicate that the biosensor designed with the thiol-labeled probe is more sensitive, and that the biosensor designed with the biotin-labeled probe has a shorter...
Tipo: Text Palavras-chave: Alexandrium Minutum; Avidin; Biosensor; Biotin; DNA; Quartz crystal miocrobalance; Self-assembled monolayer; Thiol.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00107/21810/19385.pdf
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